Construct: shRNA TRCN0000024582

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016905.1-930s1c1
DNA Barcode:
CTACTCACTCAGGGATGACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Galk1 (14635)
Gene Description:
galactokinase 1
Transcript:
RefSeq NM_016905.1 (NON-CURRENT)
Match location:
Position 930 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024582)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACTCACTCAGGGATGACTA
Hairpin Sequence:
5'-CCGG-CTACTCACTCAGGGATGACTA-CTCGAG-TAGTCATCCCTGAGTGAGTAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACTCACTCAGGGATGACTACTCGAGTAGTCATCCCTGAGTGAGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACTCACTCAGGGATGACTACTCGAGTAGTCATCCCTGAGTGAGTAG-3'

Other clones with same target sequence:

  1. TRCN0000297861