Construct: shRNA TRCN0000024626

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010293.1-468s1c1
DNA Barcode:
GCCTTCCAATCAGCACTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gykl1 (14625)
Gene Description:
glycerol kinase-like 1
Transcript:
RefSeq NM_010293.1 (NON-CURRENT)
Match location:
Position 468 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024626)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCCAATCAGCACTTATT
Hairpin Sequence:
5'-CCGG-GCCTTCCAATCAGCACTTATT-CTCGAG-AATAAGTGCTGATTGGAAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCCAATCAGCACTTATTCTCGAGAATAAGTGCTGATTGGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCCAATCAGCACTTATTCTCGAGAATAAGTGCTGATTGGAAGGC-3'

Other clones with same target sequence:

(none)