Construct: shRNA TRCN0000024627

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010293.1-140s1c1
DNA Barcode:
GCTGAATTACTTTGTCATCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gykl1 (14625)
Gene Description:
glycerol kinase-like 1
Transcript:
RefSeq NM_010293.1 (NON-CURRENT)
Match location:
Position 140 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024627)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAATTACTTTGTCATCAT
Hairpin Sequence:
5'-CCGG-GCTGAATTACTTTGTCATCAT-CTCGAG-ATGATGACAAAGTAATTCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAATTACTTTGTCATCATCTCGAGATGATGACAAAGTAATTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAATTACTTTGTCATCATCTCGAGATGATGACAAAGTAATTCAGC-3'

Other clones with same target sequence:

(none)