Construct: shRNA TRCN0000024632

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020011.3-1118s1c1
DNA Barcode:
GCTCATATTGGTCAATCCCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sphk2 (56632)
Gene Description:
sphingosine kinase 2
Transcript:
RefSeq NM_020011.3 (NON-CURRENT)
Match location:
Position 1118 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024632)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCATATTGGTCAATCCCTT
Hairpin Sequence:
5'-CCGG-GCTCATATTGGTCAATCCCTT-CTCGAG-AAGGGATTGACCAATATGAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCATATTGGTCAATCCCTTCTCGAGAAGGGATTGACCAATATGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCATATTGGTCAATCCCTTCTCGAGAAGGGATTGACCAATATGAGC-3'

Other clones with same target sequence:

(none)