Construct: shRNA TRCN0000024656

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008194.1-710s1c1
DNA Barcode:
CCATTGATTCATGGCTTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gk (14933)
Gene Description:
glycerol kinase
Transcript:
RefSeq NM_008194.1 (NON-CURRENT)
Match location:
Position 623 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024656)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTGATTCATGGCTTATTT
Hairpin Sequence:
5'-CCGG-CCATTGATTCATGGCTTATTT-CTCGAG-AAATAAGCCATGAATCAATGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTGATTCATGGCTTATTTCTCGAGAAATAAGCCATGAATCAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTGATTCATGGCTTATTTCTCGAGAAATAAGCCATGAATCAATGG-3'

Other clones with same target sequence:

  1. TRCN0000319760