Construct: shRNA TRCN0000024701

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_054097.2-683s1c1
DNA Barcode:
GCATAGGAAGTATGACCTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pip4k2c (117150)
Gene Description:
phosphatidylinositol-5-phosphate 4-kinase, type II, gamma
Transcript:
RefSeq NM_054097.2 (NON-CURRENT)
Match location:
Position 683 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024701)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATAGGAAGTATGACCTCAA
Hairpin Sequence:
5'-CCGG-GCATAGGAAGTATGACCTCAA-CTCGAG-TTGAGGTCATACTTCCTATGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATAGGAAGTATGACCTCAACTCGAGTTGAGGTCATACTTCCTATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATAGGAAGTATGACCTCAACTCGAGTTGAGGTCATACTTCCTATGC-3'

Other clones with same target sequence:

  1. TRCN0000319781