Construct: shRNA TRCN0000024740
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_283638.2-2153s1c1
- DNA Barcode:
- CGACATACATCCAGAGGACAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- LOC329248 (329248)
- Gene Description:
- similar to Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing beta polypeptide (Phosphoinositide 3-Kinase-C2-beta) (PtdIns-3-kinase C2 beta) (PI3K-C2beta) (C2-PI3K)
- Transcript:
- RefSeq XM_283638.2 (NON-CURRENT)
- Match location:
- Position 2153 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000024740)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CGACATACATCCAGAGGACAT
- Hairpin Sequence:
-
5'-CCGG-CGACATACATCCAGAGGACAT-CTCGAG-ATGTCCTCTGGATGTATGTCG-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGACATACATCCAGAGGACATCTCGAGATGTCCTCTGGATGTATGTCGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGACATACATCCAGAGGACATCTCGAGATGTCCTCTGGATGTATGTCG-3'
Other clones with same target sequence:
(none)
