Construct: shRNA TRCN0000024743

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_283638.2-1182s1c1
DNA Barcode:
CGAGAACATCAGAGTCATCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC329248 (329248)
Gene Description:
similar to Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing beta polypeptide (Phosphoinositide 3-Kinase-C2-beta) (PtdIns-3-kinase C2 beta) (PI3K-C2beta) (C2-PI3K)
Transcript:
RefSeq XM_283638.2 (NON-CURRENT)
Match location:
Position 1182 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024743)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGAACATCAGAGTCATCTT
Hairpin Sequence:
5'-CCGG-CGAGAACATCAGAGTCATCTT-CTCGAG-AAGATGACTCTGATGTTCTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGAACATCAGAGTCATCTTCTCGAGAAGATGACTCTGATGTTCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGAACATCAGAGTCATCTTCTCGAGAAGATGACTCTGATGTTCTCG-3'

Other clones with same target sequence:

(none)