Construct: shRNA TRCN0000024756

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_141596.2-2112s1c1
DNA Barcode:
CCCTGCAATCAATGATGGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm360 (245335)
Gene Description:
gene model 360, (NCBI)
Transcript:
RefSeq XM_141596.2 (NON-CURRENT)
Match location:
Position 2112 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024756)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGCAATCAATGATGGGAA
Hairpin Sequence:
5'-CCGG-CCCTGCAATCAATGATGGGAA-CTCGAG-TTCCCATCATTGATTGCAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGCAATCAATGATGGGAACTCGAGTTCCCATCATTGATTGCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGCAATCAATGATGGGAACTCGAGTTCCCATCATTGATTGCAGGG-3'

Other clones with same target sequence:

(none)