Construct: shRNA TRCN0000024792

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029094.1-1201s1c1
DNA Barcode:
CCTTTCCAAATTACCTTGGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pik3cb (74769)
Gene Description:
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta
Transcript:
RefSeq NM_029094.1 (NON-CURRENT)
Match location:
Position 1201 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024792)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTCCAAATTACCTTGGTT
Hairpin Sequence:
5'-CCGG-CCTTTCCAAATTACCTTGGTT-CTCGAG-AACCAAGGTAATTTGGAAAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTCCAAATTACCTTGGTTCTCGAGAACCAAGGTAATTTGGAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTCCAAATTACCTTGGTTCTCGAGAACCAAGGTAATTTGGAAAGG-3'

Other clones with same target sequence:

(none)