Construct: shRNA TRCN0000024802

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023538.1-1019s1c1
DNA Barcode:
CCTGATGAGCAAAGAGGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Agk (69923)
Gene Description:
acylglycerol kinase
Transcript:
RefSeq NM_023538.1 (NON-CURRENT)
Match location:
Position 1019 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024802)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGATGAGCAAAGAGGATTT
Hairpin Sequence:
5'-CCGG-CCTGATGAGCAAAGAGGATTT-CTCGAG-AAATCCTCTTTGCTCATCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGATGAGCAAAGAGGATTTCTCGAGAAATCCTCTTTGCTCATCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGATGAGCAAAGAGGATTTCTCGAGAAATCCTCTTTGCTCATCAGG-3'

Other clones with same target sequence:

(none)