Construct: shRNA TRCN0000024850

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172134.1-381s1c1
DNA Barcode:
CACTGGTTACACGAGAGACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pdxk (216134)
Gene Description:
pyridoxal (pyridoxine, vitamin B6) kinase
Transcript:
RefSeq NM_172134.1 (NON-CURRENT)
Match location:
Position 381 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024850)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTGGTTACACGAGAGACAA
Hairpin Sequence:
5'-CCGG-CACTGGTTACACGAGAGACAA-CTCGAG-TTGTCTCTCGTGTAACCAGTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTGGTTACACGAGAGACAACTCGAGTTGTCTCTCGTGTAACCAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTGGTTACACGAGAGACAACTCGAGTTGTCTCTCGTGTAACCAGTG-3'

Other clones with same target sequence:

  1. TRCN0000319788