Construct: shRNA TRCN0000024859

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_356582.1-227s1c1
DNA Barcode:
CCTCTGTGGTTATCAGAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC382588 (382588)
Gene Description:
similar to Diacylglycerol kinase, beta (Diglyceride kinase) (DGK-beta) (DAG kinase beta) (90 kDa diacylglycerol kinase)
Transcript:
RefSeq XM_356582.1 (NON-CURRENT)
Match location:
Position 227 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024859)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTGTGGTTATCAGAACAA
Hairpin Sequence:
5'-CCGG-CCTCTGTGGTTATCAGAACAA-CTCGAG-TTGTTCTGATAACCACAGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTGTGGTTATCAGAACAACTCGAGTTGTTCTGATAACCACAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTGTGGTTATCAGAACAACTCGAGTTGTTCTGATAACCACAGAGG-3'

Other clones with same target sequence:

(none)