Construct: shRNA TRCN0000024864

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025951.1-1132s1c1
DNA Barcode:
CCTGATGAATGGCGAGCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pi4k2b (67073)
Gene Description:
phosphatidylinositol 4-kinase type 2 beta
Transcript:
RefSeq NM_025951.1 (NON-CURRENT)
Match location:
Position 1132 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024864)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGATGAATGGCGAGCATAT
Hairpin Sequence:
5'-CCGG-CCTGATGAATGGCGAGCATAT-CTCGAG-ATATGCTCGCCATTCATCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGATGAATGGCGAGCATATCTCGAGATATGCTCGCCATTCATCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGATGAATGGCGAGCATATCTCGAGATATGCTCGCCATTCATCAGG-3'

Other clones with same target sequence:

(none)