Construct: shRNA TRCN0000024904

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008705.3-594s1c1
DNA Barcode:
CGAAGAACTGATCGACTACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nme2 (18103)
Gene Description:
NME/NM23 nucleoside diphosphate kinase 2
Transcript:
RefSeq NM_008705.3 (NON-CURRENT)
Match location:
Position 594 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024904)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAGAACTGATCGACTACAA
Hairpin Sequence:
5'-CCGG-CGAAGAACTGATCGACTACAA-CTCGAG-TTGTAGTCGATCAGTTCTTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAGAACTGATCGACTACAACTCGAGTTGTAGTCGATCAGTTCTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAGAACTGATCGACTACAACTCGAGTTGTAGTCGATCAGTTCTTCG-3'

Other clones with same target sequence:

  1. TRCN0000295136