Construct: shRNA TRCN0000024977

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021280.2-3575s1c1
DNA Barcode:
CCAGATCAGTAACCCAGAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plcg1 (18803)
Gene Description:
phospholipase C, gamma 1
Transcript:
RefSeq NM_021280.2 (NON-CURRENT)
Match location:
Position 3575 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000024977)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATCAGTAACCCAGAGTT
Hairpin Sequence:
5'-CCGG-CCAGATCAGTAACCCAGAGTT-CTCGAG-AACTCTGGGTTACTGATCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATCAGTAACCCAGAGTTCTCGAGAACTCTGGGTTACTGATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATCAGTAACCCAGAGTTCTCGAGAACTCTGGGTTACTGATCTGG-3'

Other clones with same target sequence:

  1. TRCN0000298133