Construct: shRNA TRCN0000025000

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_127605.4-2000s1c1
DNA Barcode:
GCATTGCTGATGGTCGCTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dlg5 (71228)
Gene Description:
discs, large homolog 5 (Drosophila)
Transcript:
RefSeq XM_127605.4 (NON-CURRENT)
Match location:
Position 2000 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025000)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTGCTGATGGTCGCTTAA
Hairpin Sequence:
5'-CCGG-GCATTGCTGATGGTCGCTTAA-CTCGAG-TTAAGCGACCATCAGCAATGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTGCTGATGGTCGCTTAACTCGAGTTAAGCGACCATCAGCAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTGCTGATGGTCGCTTAACTCGAGTTAAGCGACCATCAGCAATGC-3'

Other clones with same target sequence:

  1. TRCN0000339145