Construct: shRNA TRCN0000025008

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_131496.4-660s1c1
DNA Barcode:
CCCAGACTTCTTATCATGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fggy (75578)
Gene Description:
FGGY carbohydrate kinase domain containing
Transcript:
RefSeq XM_131496.4 (NON-CURRENT)
Match location:
Position 660 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025008)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGACTTCTTATCATGGAA
Hairpin Sequence:
5'-CCGG-CCCAGACTTCTTATCATGGAA-CTCGAG-TTCCATGATAAGAAGTCTGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGACTTCTTATCATGGAACTCGAGTTCCATGATAAGAAGTCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGACTTCTTATCATGGAACTCGAGTTCCATGATAAGAAGTCTGGG-3'

Other clones with same target sequence:

(none)