Construct: shRNA TRCN0000025021

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_136225.3-1153s1c1
DNA Barcode:
CGATGGCATCAATGATGCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pik3c2b (240752)
Gene Description:
phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta
Transcript:
RefSeq XM_136225.3 (NON-CURRENT)
Match location:
Position 1153 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025021)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATGGCATCAATGATGCAAT
Hairpin Sequence:
5'-CCGG-CGATGGCATCAATGATGCAAT-CTCGAG-ATTGCATCATTGATGCCATCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATGGCATCAATGATGCAATCTCGAGATTGCATCATTGATGCCATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATGGCATCAATGATGCAATCTCGAGATTGCATCATTGATGCCATCG-3'

Other clones with same target sequence:

(none)