Construct: shRNA TRCN0000025101

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198191.1-742s1c1
DNA Barcode:
GTATGACATCAAGGGCTGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pip5kl1 (227733)
Gene Description:
phosphatidylinositol-4-phosphate 5-kinase-like 1
Transcript:
RefSeq NM_198191.1 (NON-CURRENT)
Match location:
Position 742 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025101)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATGACATCAAGGGCTGCAA
Hairpin Sequence:
5'-CCGG-GTATGACATCAAGGGCTGCAA-CTCGAG-TTGCAGCCCTTGATGTCATAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATGACATCAAGGGCTGCAACTCGAGTTGCAGCCCTTGATGTCATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATGACATCAAGGGCTGCAACTCGAGTTGCAGCCCTTGATGTCATAC-3'

Other clones with same target sequence:

(none)