Construct: shRNA TRCN0000025122

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153744.1-85s1c1
DNA Barcode:
GAGATGGACTTCTTAGAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prkag3 (241113)
Gene Description:
protein kinase, AMP-activated, gamma 3 non-catatlytic subunit
Transcript:
RefSeq NM_153744.1 (NON-CURRENT)
Match location:
Position 85 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025122)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGATGGACTTCTTAGAACAA
Hairpin Sequence:
5'-CCGG-GAGATGGACTTCTTAGAACAA-CTCGAG-TTGTTCTAAGAAGTCCATCTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGATGGACTTCTTAGAACAACTCGAGTTGTTCTAAGAAGTCCATCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGATGGACTTCTTAGAACAACTCGAGTTGTTCTAAGAAGTCCATCTC-3'

Other clones with same target sequence:

(none)