Construct: shRNA TRCN0000025163
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_016693.2-3939s1c1
- DNA Barcode:
- CTACAGGAACTGAGTGTAGAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Map3k6 (53608)
- Gene Description:
- mitogen-activated protein kinase kinase kinase 6
- Transcript:
- RefSeq NM_016693.2 (NON-CURRENT)
- Match location:
- Position 3939 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000025163)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CTACAGGAACTGAGTGTAGAT
- Hairpin Sequence:
-
5'-CCGG-CTACAGGAACTGAGTGTAGAT-CTCGAG-ATCTACACTCAGTTCCTGTAG-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCTACAGGAACTGAGTGTAGATCTCGAGATCTACACTCAGTTCCTGTAGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACTACAGGAACTGAGTGTAGATCTCGAGATCTACACTCAGTTCCTGTAG-3'
Other clones with same target sequence:
-
TRCN0000339088
