Construct: shRNA TRCN0000025179

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011945.1-4267s1c1
DNA Barcode:
GCGCCATTATAGAAATGGCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Map3k1 (26401)
Gene Description:
mitogen-activated protein kinase kinase kinase 1
Transcript:
RefSeq NM_011945.1 (NON-CURRENT)
Match location:
Position 4267 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025179)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCCATTATAGAAATGGCTT
Hairpin Sequence:
5'-CCGG-GCGCCATTATAGAAATGGCTT-CTCGAG-AAGCCATTTCTATAATGGCGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCCATTATAGAAATGGCTTCTCGAGAAGCCATTTCTATAATGGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCCATTATAGAAATGGCTTCTCGAGAAGCCATTTCTATAATGGCGC-3'

Other clones with same target sequence:

(none)