Construct: shRNA TRCN0000025207

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008696.1-248s1c1
DNA Barcode:
CGTACTATGGTGCTTTCATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Map4k4 (26921)
Gene Description:
mitogen-activated protein kinase kinase kinase kinase 4
Transcript:
RefSeq NM_008696.1 (NON-CURRENT)
Match location:
Position 248 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025207)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTACTATGGTGCTTTCATTA
Hairpin Sequence:
5'-CCGG-CGTACTATGGTGCTTTCATTA-CTCGAG-TAATGAAAGCACCATAGTACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTACTATGGTGCTTTCATTACTCGAGTAATGAAAGCACCATAGTACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTACTATGGTGCTTTCATTACTCGAGTAATGAAAGCACCATAGTACG-3'

Other clones with same target sequence:

  1. TRCN0000322214