Construct: shRNA TRCN0000025212

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177326.1-900s1c1
DNA Barcode:
GAGTTAAAGAATCCCAACATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pak2 (224105)
Gene Description:
p21 protein (Cdc42/Rac)-activated kinase 2
Transcript:
RefSeq NM_177326.1 (NON-CURRENT)
Match location:
Position 900 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025212)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTTAAAGAATCCCAACATA
Hairpin Sequence:
5'-CCGG-GAGTTAAAGAATCCCAACATA-CTCGAG-TATGTTGGGATTCTTTAACTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTTAAAGAATCCCAACATACTCGAGTATGTTGGGATTCTTTAACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTTAAAGAATCCCAACATACTCGAGTATGTTGGGATTCTTTAACTC-3'

Other clones with same target sequence:

(none)