Construct: shRNA TRCN0000025221

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_355941.1-2008s1c1
DNA Barcode:
CGAGAGGACTTGAATAAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Taok2 (381921)
Gene Description:
TAO kinase 2
Transcript:
RefSeq XM_355941.1 (NON-CURRENT)
Match location:
Position 2008 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025221)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGAGGACTTGAATAAGAAA
Hairpin Sequence:
5'-CCGG-CGAGAGGACTTGAATAAGAAA-CTCGAG-TTTCTTATTCAAGTCCTCTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGAGGACTTGAATAAGAAACTCGAGTTTCTTATTCAAGTCCTCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGAGGACTTGAATAAGAAACTCGAGTTTCTTATTCAAGTCCTCTCG-3'

Other clones with same target sequence:

(none)