Construct: shRNA TRCN0000025223

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_355941.1-3374s1c1
DNA Barcode:
CCAATAAGAATGGTTTCCGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Taok2 (381921)
Gene Description:
TAO kinase 2
Transcript:
RefSeq XM_355941.1 (NON-CURRENT)
Match location:
Position 3374 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025223)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAATAAGAATGGTTTCCGAA
Hairpin Sequence:
5'-CCGG-CCAATAAGAATGGTTTCCGAA-CTCGAG-TTCGGAAACCATTCTTATTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAATAAGAATGGTTTCCGAACTCGAGTTCGGAAACCATTCTTATTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAATAAGAATGGTTTCCGAACTCGAGTTCGGAAACCATTCTTATTGG-3'

Other clones with same target sequence:

(none)