Construct: shRNA TRCN0000025236

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011840.1-1853s1c1
DNA Barcode:
CGTGCAGTTCAATGATGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Map2k5 (23938)
Gene Description:
mitogen-activated protein kinase kinase 5
Transcript:
RefSeq NM_011840.1 (NON-CURRENT)
Match location:
Position 1853 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025236)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGCAGTTCAATGATGGAAA
Hairpin Sequence:
5'-CCGG-CGTGCAGTTCAATGATGGAAA-CTCGAG-TTTCCATCATTGAACTGCACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGCAGTTCAATGATGGAAACTCGAGTTTCCATCATTGAACTGCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGCAGTTCAATGATGGAAACTCGAGTTTCCATCATTGAACTGCACG-3'

Other clones with same target sequence:

  1. TRCN0000322119