Construct: shRNA TRCN0000025241

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145465.1-459s1c1
DNA Barcode:
GCCTGGCCCTTTAGATGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stk24 (223255)
Gene Description:
serine/threonine kinase 24
Transcript:
RefSeq NM_145465.1 (NON-CURRENT)
Match location:
Position 459 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025241)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGGCCCTTTAGATGAAAT
Hairpin Sequence:
5'-CCGG-GCCTGGCCCTTTAGATGAAAT-CTCGAG-ATTTCATCTAAAGGGCCAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGGCCCTTTAGATGAAATCTCGAGATTTCATCTAAAGGGCCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGGCCCTTTAGATGAAATCTCGAGATTTCATCTAAAGGGCCAGGC-3'

Other clones with same target sequence:

  1. TRCN0000276725