Construct: shRNA TRCN0000025264

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009157.2-223s1c1
DNA Barcode:
CGGTTTACTCTGAATCCTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Map2k4 (26398)
Gene Description:
mitogen-activated protein kinase kinase 4
Transcript:
RefSeq NM_009157.2 (NON-CURRENT)
Match location:
Position 223 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025264)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTTTACTCTGAATCCTAAT
Hairpin Sequence:
5'-CCGG-CGGTTTACTCTGAATCCTAAT-CTCGAG-ATTAGGATTCAGAGTAAACCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTTTACTCTGAATCCTAATCTCGAGATTAGGATTCAGAGTAAACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTTTACTCTGAATCCTAATCTCGAGATTAGGATTCAGAGTAAACCG-3'

Other clones with same target sequence:

  1. TRCN0000345129