Construct: shRNA TRCN0000025310

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_136210.4-496s1c1
DNA Barcode:
CCGTTCATATTGGCTAGGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC210381 (210381)
Gene Description:
similar to FLJ23074 protein
Transcript:
RefSeq XM_136210.4 (NON-CURRENT)
Match location:
Position 496 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025310)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTTCATATTGGCTAGGGAA
Hairpin Sequence:
5'-CCGG-CCGTTCATATTGGCTAGGGAA-CTCGAG-TTCCCTAGCCAATATGAACGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTTCATATTGGCTAGGGAACTCGAGTTCCCTAGCCAATATGAACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTTCATATTGGCTAGGGAACTCGAGTTCCCTAGCCAATATGAACGG-3'

Other clones with same target sequence:

(none)