Construct: shRNA TRCN0000025326

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_135264.4-1879s1c1
DNA Barcode:
GATGGCAAACTGATAGGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Oxsr1 (108737)
Gene Description:
oxidative-stress responsive 1
Transcript:
RefSeq XM_135264.4 (NON-CURRENT)
Match location:
Position 1879 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025326)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGGCAAACTGATAGGATTT
Hairpin Sequence:
5'-CCGG-GATGGCAAACTGATAGGATTT-CTCGAG-AAATCCTATCAGTTTGCCATC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGGCAAACTGATAGGATTTCTCGAGAAATCCTATCAGTTTGCCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGGCAAACTGATAGGATTTCTCGAGAAATCCTATCAGTTTGCCATC-3'

Other clones with same target sequence:

  1. TRCN0000319754