Construct: shRNA TRCN0000025351

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145496.1-817s1c1
DNA Barcode:
CCACATGACAAACACCTCCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tkfc (225913)
Gene Description:
triokinase, FMN cyclase
Transcript:
RefSeq NM_145496.1 (CURRENT)
Match location:
Position 817 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025351)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACATGACAAACACCTCCAA
Hairpin Sequence:
5'-CCGG-CCACATGACAAACACCTCCAA-CTCGAG-TTGGAGGTGTTTGTCATGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACATGACAAACACCTCCAACTCGAGTTGGAGGTGTTTGTCATGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACATGACAAACACCTCCAACTCGAGTTGGAGGTGTTTGTCATGTGG-3'

Other clones with same target sequence:

(none)