Construct: shRNA TRCN0000025535

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013764.1-568s1c1
DNA Barcode:
GCTTCATCTATCTCCAGGCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dguok (27369)
Gene Description:
deoxyguanosine kinase
Transcript:
RefSeq NM_013764.1 (NON-CURRENT)
Match location:
Position 568 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025535)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCATCTATCTCCAGGCTT
Hairpin Sequence:
5'-CCGG-GCTTCATCTATCTCCAGGCTT-CTCGAG-AAGCCTGGAGATAGATGAAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCATCTATCTCCAGGCTTCTCGAGAAGCCTGGAGATAGATGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCATCTATCTCCAGGCTTCTCGAGAAGCCTGGAGATAGATGAAGC-3'

Other clones with same target sequence:

  1. TRCN0000278666