Construct: shRNA TRCN0000025539

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026662.2-830s1c1
DNA Barcode:
GCCACCAAAGTTTATGCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prps2 (110639)
Gene Description:
phosphoribosyl pyrophosphate synthetase 2
Transcript:
RefSeq NM_026662.2 (NON-CURRENT)
Match location:
Position 830 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025539)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACCAAAGTTTATGCTATT
Hairpin Sequence:
5'-CCGG-GCCACCAAAGTTTATGCTATT-CTCGAG-AATAGCATAAACTTTGGTGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACCAAAGTTTATGCTATTCTCGAGAATAGCATAAACTTTGGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACCAAAGTTTATGCTATTCTCGAGAATAGCATAAACTTTGGTGGC-3'

Other clones with same target sequence:

(none)