Construct: shRNA TRCN0000025552

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_356581.1-574s1c1
DNA Barcode:
CAAGACCTGTTGTCAAGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC382587 (382587)
Gene Description:
similar to Diacylglycerol kinase, beta (Diglyceride kinase) (DGK-beta) (DAG kinase beta) (90 kDa diacylglycerol kinase)
Transcript:
RefSeq XM_356581.1 (NON-CURRENT)
Match location:
Position 574 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025552)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGACCTGTTGTCAAGTGAA
Hairpin Sequence:
5'-CCGG-CAAGACCTGTTGTCAAGTGAA-CTCGAG-TTCACTTGACAACAGGTCTTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGACCTGTTGTCAAGTGAACTCGAGTTCACTTGACAACAGGTCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGACCTGTTGTCAAGTGAACTCGAGTTCACTTGACAACAGGTCTTG-3'

Other clones with same target sequence:

(none)