Construct: shRNA TRCN0000025576

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008845.2-381s1c1
DNA Barcode:
GCCGAGCCATTTCAAGTTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pip4k2a (18718)
Gene Description:
phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
Transcript:
RefSeq NM_008845.2 (NON-CURRENT)
Match location:
Position 381 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025576)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGAGCCATTTCAAGTTTAA
Hairpin Sequence:
5'-CCGG-GCCGAGCCATTTCAAGTTTAA-CTCGAG-TTAAACTTGAAATGGCTCGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGAGCCATTTCAAGTTTAACTCGAGTTAAACTTGAAATGGCTCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGAGCCATTTCAAGTTTAACTCGAGTTAAACTTGAAATGGCTCGGC-3'

Other clones with same target sequence:

(none)