Construct: shRNA TRCN0000025611

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_149165.3-1707s1c1
DNA Barcode:
CCAAGACTGATCAGGCTCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cerkl (228094)
Gene Description:
ceramide kinase-like
Transcript:
RefSeq XM_149165.3 (NON-CURRENT)
Match location:
Position 1707 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025611)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGACTGATCAGGCTCTAT
Hairpin Sequence:
5'-CCGG-CCAAGACTGATCAGGCTCTAT-CTCGAG-ATAGAGCCTGATCAGTCTTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGACTGATCAGGCTCTATCTCGAGATAGAGCCTGATCAGTCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGACTGATCAGGCTCTATCTCGAGATAGAGCCTGATCAGTCTTGG-3'

Other clones with same target sequence:

(none)