Construct: shRNA TRCN0000025612

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_149165.3-1029s1c1
DNA Barcode:
GCAACCATGCATATTATACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cerkl (228094)
Gene Description:
ceramide kinase-like
Transcript:
RefSeq XM_149165.3 (NON-CURRENT)
Match location:
Position 1029 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025612)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACCATGCATATTATACTA
Hairpin Sequence:
5'-CCGG-GCAACCATGCATATTATACTA-CTCGAG-TAGTATAATATGCATGGTTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACCATGCATATTATACTACTCGAGTAGTATAATATGCATGGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACCATGCATATTATACTACTCGAGTAGTATAATATGCATGGTTGC-3'

Other clones with same target sequence:

(none)