Construct: shRNA TRCN0000025625

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_284750.2-1129s1c1
DNA Barcode:
CCAGTTATAATGGAGCTAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pfkfb1 (18639)
Gene Description:
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
Transcript:
RefSeq XM_284750.2 (NON-CURRENT)
Match location:
Position 1129 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025625)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTTATAATGGAGCTAGAA
Hairpin Sequence:
5'-CCGG-CCAGTTATAATGGAGCTAGAA-CTCGAG-TTCTAGCTCCATTATAACTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTTATAATGGAGCTAGAACTCGAGTTCTAGCTCCATTATAACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTTATAATGGAGCTAGAACTCGAGTTCTAGCTCCATTATAACTGG-3'

Other clones with same target sequence:

(none)