Construct: shRNA TRCN0000025691

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028776.3-2181s1c1
DNA Barcode:
GCAGATATGATCCCAGAAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Scyl3 (240880)
Gene Description:
SCY1-like 3 (S. cerevisiae)
Transcript:
RefSeq NM_028776.3 (NON-CURRENT)
Match location:
Position 2181 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025691)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATATGATCCCAGAAATT
Hairpin Sequence:
5'-CCGG-GCAGATATGATCCCAGAAATT-CTCGAG-AATTTCTGGGATCATATCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATATGATCCCAGAAATTCTCGAGAATTTCTGGGATCATATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATATGATCCCAGAAATTCTCGAGAATTTCTGGGATCATATCTGC-3'

Other clones with same target sequence:

(none)