Construct: shRNA TRCN0000025740

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010130.1-1211s1c1
DNA Barcode:
GCAGGGATCTTGGTTATGCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adgre1 (13733)
Gene Description:
adhesion G protein-coupled receptor E1
Transcript:
RefSeq NM_010130.1 (NON-CURRENT)
Match location:
Position 1211 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025740)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGGATCTTGGTTATGCTT
Hairpin Sequence:
5'-CCGG-GCAGGGATCTTGGTTATGCTT-CTCGAG-AAGCATAACCAAGATCCCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGGATCTTGGTTATGCTTCTCGAGAAGCATAACCAAGATCCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGGATCTTGGTTATGCTTCTCGAGAAGCATAACCAAGATCCCTGC-3'

Other clones with same target sequence:

(none)