Construct: shRNA TRCN0000025937

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019483.2-1084s1c1
DNA Barcode:
CACGGCTTTGAAGTGGTGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smad9 (55994)
Gene Description:
SMAD family member 9
Transcript:
RefSeq NM_019483.2 (NON-CURRENT)
Match location:
Position 1084 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025937)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACGGCTTTGAAGTGGTGTAT
Hairpin Sequence:
5'-CCGG-CACGGCTTTGAAGTGGTGTAT-CTCGAG-ATACACCACTTCAAAGCCGTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACGGCTTTGAAGTGGTGTATCTCGAGATACACCACTTCAAAGCCGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACGGCTTTGAAGTGGTGTATCTCGAGATACACCACTTCAAAGCCGTG-3'

Other clones with same target sequence:

(none)