Construct: shRNA TRCN0000025976

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010444.1-729s1c1
DNA Barcode:
CCAGAGTTCTCTGAAATTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nr4a1 (15370)
Gene Description:
nuclear receptor subfamily 4, group A, member 1
Transcript:
RefSeq NM_010444.1 (NON-CURRENT)
Match location:
Position 729 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025976)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAGTTCTCTGAAATTGTT
Hairpin Sequence:
5'-CCGG-CCAGAGTTCTCTGAAATTGTT-CTCGAG-AACAATTTCAGAGAACTCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAGTTCTCTGAAATTGTTCTCGAGAACAATTTCAGAGAACTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAGTTCTCTGAAATTGTTCTCGAGAACAATTTCAGAGAACTCTGG-3'

Other clones with same target sequence:

(none)