Construct: shRNA TRCN0000025992

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152229.1-1447s1c1
DNA Barcode:
CCAGAAGCTGAACAAGATCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nr2e1 (21907)
Gene Description:
nuclear receptor subfamily 2, group E, member 1
Transcript:
RefSeq NM_152229.1 (NON-CURRENT)
Match location:
Position 1447 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000025992)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAAGCTGAACAAGATCAT
Hairpin Sequence:
5'-CCGG-CCAGAAGCTGAACAAGATCAT-CTCGAG-ATGATCTTGTTCAGCTTCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAAGCTGAACAAGATCATCTCGAGATGATCTTGTTCAGCTTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAAGCTGAACAAGATCATCTCGAGATGATCTTGTTCAGCTTCTGG-3'

Other clones with same target sequence:

(none)