Construct: shRNA TRCN0000026021

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011145.1-414s1c1
DNA Barcode:
GAACCGCAACAAGTGTCAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppard (19015)
Gene Description:
peroxisome proliferator activator receptor delta
Transcript:
RefSeq NM_011145.1 (NON-CURRENT)
Match location:
Position 414 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000026021)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACCGCAACAAGTGTCAGTA
Hairpin Sequence:
5'-CCGG-GAACCGCAACAAGTGTCAGTA-CTCGAG-TACTGACACTTGTTGCGGTTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACCGCAACAAGTGTCAGTACTCGAGTACTGACACTTGTTGCGGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACCGCAACAAGTGTCAGTACTCGAGTACTGACACTTGTTGCGGTTC-3'

Other clones with same target sequence:

(none)