Construct: shRNA TRCN0000026051

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_356093.1-2808s1c1
DNA Barcode:
CCAAGGTACTTCCAGGATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nr3c2 (110784)
Gene Description:
nuclear receptor subfamily 3, group C, member 2
Transcript:
RefSeq XM_356093.1 (NON-CURRENT)
Match location:
Position 2808 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000026051)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGGTACTTCCAGGATTTA
Hairpin Sequence:
5'-CCGG-CCAAGGTACTTCCAGGATTTA-CTCGAG-TAAATCCTGGAAGTACCTTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGGTACTTCCAGGATTTACTCGAGTAAATCCTGGAAGTACCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGGTACTTCCAGGATTTACTCGAGTAAATCCTGGAAGTACCTTGG-3'

Other clones with same target sequence:

(none)