Construct: shRNA TRCN0000026106

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175493.2-462s1c1
DNA Barcode:
CTTCGGGTACTTGCAGATCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpr68 (238377)
Gene Description:
G protein-coupled receptor 68
Transcript:
RefSeq NM_175493.2 (NON-CURRENT)
Match location:
Position 462 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000026106)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCGGGTACTTGCAGATCAA
Hairpin Sequence:
5'-CCGG-CTTCGGGTACTTGCAGATCAA-CTCGAG-TTGATCTGCAAGTACCCGAAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCGGGTACTTGCAGATCAACTCGAGTTGATCTGCAAGTACCCGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCGGGTACTTGCAGATCAACTCGAGTTGATCTGCAAGTACCCGAAG-3'

Other clones with same target sequence:

(none)