Construct: shRNA TRCN0000026114

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134438.1-1014s1c1
DNA Barcode:
CTGTGGTCTATGCCTTCTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpr37l1 (171469)
Gene Description:
G protein-coupled receptor 37-like 1
Transcript:
RefSeq NM_134438.1 (NON-CURRENT)
Match location:
Position 1014 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000026114)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTGGTCTATGCCTTCTGTA
Hairpin Sequence:
5'-CCGG-CTGTGGTCTATGCCTTCTGTA-CTCGAG-TACAGAAGGCATAGACCACAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTGGTCTATGCCTTCTGTACTCGAGTACAGAAGGCATAGACCACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTGGTCTATGCCTTCTGTACTCGAGTACAGAAGGCATAGACCACAG-3'

Other clones with same target sequence:

(none)