Construct: shRNA TRCN0000026213

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008261.2-1313s1c1
DNA Barcode:
GCACCAATGTCATTGTTGCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hnf4a (15378)
Gene Description:
hepatic nuclear factor 4, alpha
Transcript:
RefSeq NM_008261.2 (NON-CURRENT)
Match location:
Position 1313 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000026213)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCAATGTCATTGTTGCTA
Hairpin Sequence:
5'-CCGG-GCACCAATGTCATTGTTGCTA-CTCGAG-TAGCAACAATGACATTGGTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCAATGTCATTGTTGCTACTCGAGTAGCAACAATGACATTGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCAATGTCATTGTTGCTACTCGAGTAGCAACAATGACATTGGTGC-3'

Other clones with same target sequence:

(none)